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Investors / United States / 2Flo Ventures

2Flo Ventures

Venture capital · Chicago, IL, United States · North America

2floventures.comTakes pitches from founders

2Flo Ventures is a startup studio and venture capital firm focused on healthcare and life sciences. It invests in and supports companies addressing large unmet medical needs.

Invests inHealthcare & Biotech

In its own wordshealthcarelife scienceshealth equitytherapeuticsdigital health

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8portfolio companies
0funding rounds on record

2Flo Ventures lists 8 checked portfolio companies on its own site. Its largest theme is Healthcare & Biotech (100%). 100% of its companies are based in United States. It invests most often alongside HealthX Ventures, 8VC and Bold Capital Partners.

No funding round on record names 2Flo Ventures yet, so the figures come from its portfolio page.

Investor profile

percentile among all firms
Portfolio size: 8 companiesRecent activity: 0 rounds in 12 monthsEarly stage: 0% of entries at pre-seed or seedEvidence: 0% of companies confirmed by a roundSector focus: 100% in its largest sectorReach: 1 countryPortfolio size15th pctRecent activity46th pctEarly stage54th pctEvidence52th pctSector focus100th pctReach19th pct
This firmMedian of all firms
Show as a table
2Flo Ventures compared with all firms
MeasurePercentile of all firmsValue
Portfolio size15th8 companies
Recent activity46th0 rounds in 12 months
Early stage54th0% of entries at pre-seed or seed
Evidence52th0% of companies confirmed by a round
Sector focus100th100% in its largest sector
Reach19th1 country

Its portfolio companies

where they are based, and what their own sites show

Headquarters

Site status

  • Site answers8100%
  • No longer answers00%
  • Points to another company00%
  • Parked or sold domain00%

A site that stops answering three checks in a row is counted as no longer answering; that is not proof of closure. A site that points to another company is often an acquisition.

Investment themes

share of its checked companies

This firmAll checked companies

Show as a table
Investment themes
This firmAll checked companies
Healthcare & Biotech100%15%

Compare 2Flo Ventures head to head

portfolio size, rounds, entry stages, themes and the companies both back

Three or more firms at once: portfolio overlap.

Portfolio

8 companies checked against their own sites
CompanyWhat it doesThemesFirst round with itEvidence
4D Healthware4dhealthware.com · United States4D Healthware provides a virtual care management technology platform for healthcare providers to deliver care remotely
Healthcare & Biotech
Portfolio page
Akan Biosciencesakanbio.comAkan Biosciences is a biotechnology and cellular therapy company developing next-generation injectable products to treat degenerative diseases and injuries.
Healthcare & Biotech
Portfolio page
CancerIQcanceriq.com · United StatesCancerIQ helps clinicians identify patients who qualify for screening and diagnostic tests and guide them to completion
Healthcare & Biotech
Portfolio page
Delphian Therapeuticsdelphiantx.comDelphian Therapeutics is a clinical-stage company that unlocks the full therapeutic potential of optimized, multi-modal endocannabinoid system targeting to treat complex pain and CNS disorders.
Healthcare & Biotech
Portfolio page
KeyCarekeycare.org · United StatesKeyCare is a national virtual care organization built natively within Epic, giving health systems and plans a seamless way to extend access to high-quality, coordinated care.
Healthcare & Biotech
Portfolio page
ResQ Pharmaresqpharma.com · United StatesResQ Pharma develops LipidRescue, an infusion of lipid emulsion to treat toxicity caused by lipid soluble drugs.
Healthcare & Biotech
Portfolio page
Temprian Therapeuticstempriantherapeutics.com · United StatesTemprian Therapeutics is developing a novel treatment for vitiligo.
Healthcare & Biotech
Portfolio page
Vivacelle Biovivacellebio.com · United StatesVivacelle Bio develops nanoparticle-based therapies for critical care, particularly for septic shock and acute hemorrhage.
Healthcare & Biotech
Portfolio page

About this data

site last read 5 Nov 2026
The homepage of 2Flo Ventures, as last read
2floventures.com as VCinfo last read it
Site
2floventures.com
Last read
5 Nov 2026
Watching since
5 Nov 2026
Companies checked
8

Rates come from what VCinfo has observed since observation_start. Firms tend to remove companies that closed, so early rates understate reality. A company leaving a portfolio page does not mean it closed or exited. An unreachable site means our checks failed, nothing more.

How we grade the data

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