Investors / United States / Act One Ventures
Act One Ventures
Venture capital · San Francisco, CA, United States · North America
Act One Ventures is an AI-native venture firm based in San Francisco that makes first checks at pre-seed and seed. It backs founders re-architecting the enterprise for the AI economy and supports them with an in-house agent fleet built on its sourcing and decision history.
Invests inAI & Machine Learning
In its own wordsaienterpriseagentsai-nativeb2b softwarevoice ai
Your fit with Act One Ventures
Four questions about your startup
See how your round matches what it backs, point by point.
Or find every investor that fitsAct One Ventures lists 47 checked portfolio companies on its own site and is named in 3 funding rounds. Its most frequent entry is Seed, 67% of the companies with a known first round. Its largest themes are AI & Machine Learning (27%), Healthcare & Biotech (15%) and Fintech (14%). 95% of its companies are based in United States. It invests most often alongside Y Combinator, Techstars and Transpose Platform.
Counts come from funding rounds that name Act One Ventures, complete from mid-2025: earlier rounds appear only when they were reported later. Round amounts and full round histories are on Fundup AI.
Round figures show recent activity, not Act One Ventures' whole history. Rounds on record name 3 companies; its own site lists 47. Funding rounds are complete from mid-2025, so counts and the yearly trend undercount a firm that invested before then. The portfolio, themes and places come from its own site and are not affected.
Entry stage
share of companies by the stage of its first roundShow as a table
| Value | |
|---|---|
| Pre-seed | 33% |
| Seed | 67% |
| Series A | 0% |
| Series B | 0% |
| Growth | 0% |
Activity by year
rounds it joined, last 10 yearsShow as a table
| Total | Rounds it led | |
|---|---|---|
| '17 | 0 | 0 |
| '18 | 0 | 0 |
| '19 | 0 | 0 |
| '20 | 0 | 0 |
| '21 | 0 | 0 |
| '22 | 0 | 0 |
| '23 | 0 | 0 |
| '24 | 0 | 0 |
| '25 | 0 | 0 |
| '26 | 3 | 0 |
Investor profile
percentile among all firmsShow as a table
| Measure | Percentile of all firms | Value |
|---|---|---|
| Portfolio size | 82th | 47 companies |
| Recent activity | 76th | 3 rounds in 12 months |
| Early stage | 100th | 100% of entries at pre-seed or seed |
| Evidence | 68th | 6% of companies confirmed by a round |
| Sector focus | 42th | 27% in its largest sector |
| Reach | 42th | 2 countries |
Investment themes
share of its checked companiesThis firmAll checked companies
Show as a table
| This firm | All checked companies | |
|---|---|---|
| AI & Machine Learning | 27% | 18% |
| Healthcare & Biotech | 15% | 15% |
| Fintech | 14% | 12% |
| Enterprise Software | 12% | 8% |
| Cybersecurity | 7% | 4% |
| E-commerce & Marketplaces | 6% | 6% |
| Industrial & Manufacturing | 5% | 7% |
| Space & Defense | 4% | 3% |
Invests most often with
firms sharing its portfolio companiesShow as a table
| Share | |
|---|---|
| Y Combinator | 6 |
| Techstars | 3 |
| Transpose Platform | 3 |
| Amplify.LA | 2 |
| Battery Ventures | 2 |
| Collide Capital | 2 |
| Correlation Ventures | 2 |
| Insight Partners | 2 |
Its portfolio companies
where they are based, and what their own sites showSite status
- Site answers47100%
- No longer answers00%
- Points to another company00%
- Parked or sold domain00%
A site that stops answering three checks in a row is counted as no longer answering; that is not proof of closure. A site that points to another company is often an acquisition.
Co-investor network
who it backs companies with, and who they back togetherCompare Act One Ventures head to head
portfolio size, rounds, entry stages, themes and the companies both backThree or more firms at once: portfolio overlap.
Recent portfolio activity
when companies last raised with Act One Ventures| Date | Company | Stage |
|---|---|---|
| 4 Nov 2026 | Seed | |
| 8 Sept 2026 | Seed | |
| 16 Jul 2026 | Pre-Seed |
Round details for Act One Ventures
Amounts, full round histories and every co-investor in Act One Ventures' rounds are on Fundup AI. VCinfo covers investors and their portfolios.
Portfolio
47 companies checked against their own sites| Company | What it does | Themes | First round with it | Evidence |
|---|---|---|---|---|
| AcquiOS provides full-service support for private practice optometrists | Healthcare & Biotech | Portfolio page | ||
| The company provides an open-source multi-agent AI framework for building and orchestrating systems of AI agents | AI & Machine LearningEnterprise Software | Portfolio page | ||
| Aiva provides a nurse assistant that uses voice and text control to manage various hospital tools and systems. | Healthcare & Biotech | Portfolio page | ||
| Alike provides a shared environment for agents to work together, enabling swarm intelligence and multiplayer AI capabilities. | AI & Machine LearningCybersecurity | Portfolio page | ||
| Arden develops AI and hardware to automate and optimize manufacturing plants | Industrial & ManufacturingAI & Machine Learning | Portfolio page | ||
| The company provides a GRC Intelligence Platform for audit, risk, and compliance management | FintechCybersecurity | Portfolio page | ||
| Autostep uncovers repetitive tasks ready for AI and their hidden cost, then finds or builds the right agent or process change. | AI & Machine LearningEnterprise Software | Portfolio page | ||
| Blaze provides a cannabis retail platform for dispensaries that handles point of sale, e-commerce, payments, and marketing | Crypto & Web3Healthcare & Biotech | Portfolio page | ||
| BlockRun measures and settles payments made by agents | AI & Machine LearningFintech | Portfolio page | ||
| Bloomlife provides remote monitoring services for high-risk pregnancies | Healthcare & Biotech | Portfolio page | ||
| Bluecore Energy develops floating nuclear power plants for ports and cities | EducationIndustrial & Manufacturing | Sept 2026Seed | Page + round | |
| The company provides financial services and payment solutions for workforces, including earned wage access, cashless tips, and contractor payouts. | FintechFintech | Portfolio page | ||
| Chord provides an analytics platform that manages and improves context, applies permissions, and validates queries to help businesses get reliable answers from their data using AI. | AI & Machine LearningEnterprise Software | Portfolio page | ||
| Portfolio page | ||||
| Cognarus helps hospitals maintain staffing efficiency and reduce avoidable labor costs by establishing and tracking staffing standards | Healthcare & Biotech | Portfolio page | ||
| Dragonboat turns operating reality into a living system, connecting strategy, portfolio, product, delivery, and outcomes in one operating context. | Enterprise SoftwareAI & Machine Learning | Portfolio page | ||
| Empwr provides AI project management for cross-functional teams | AI & Machine LearningEnterprise Software | Portfolio page | ||
| Finix provides payment solutions for businesses of all sizes and industries | FintechE-commerce & Marketplaces | Portfolio page | ||
| Flossy provides AI workforce solutions for businesses to handle calls, automate operations, and grow their business | Healthcare & BiotechAI & Machine Learning | Portfolio page | ||
| Portfolio page |
About this data
site last read 5 Nov 2026- Site
- actoneventures.com
- Last read
- 5 Nov 2026
- Watching since
- 5 Nov 2026
- Rounds since
- 16 Jul 2026
- Companies checked
- 47
Rates come from what VCinfo has observed since observation_start. Firms tend to remove companies that closed, so early rates understate reality. A company leaving a portfolio page does not mean it closed or exited. An unreachable site means our checks failed, nothing more.
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